FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n02_3033_d3.352000000be260.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n02_3033_d3.352000000be260.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17949979
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG273640.15244586080017142No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC227750.1268803712806572No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC192610.1073037467063332No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA185670.10343744691846156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT72650.040.289871
AACCGTG77450.037.173812
ACCGTGT99450.029.092933
CCGTGTT111050.026.1817174
CGTGTTC124650.023.3603295
TTCACTA158600.018.530669
TGTTCAC180200.017.1278177
GTTCACT174750.017.0898448
AACTCAC157700.016.1150935
ACTCACC162550.015.9204056
GTGTTCA194350.015.7831116
CGCGAAT4904.620233E-1014.5934621
ATTAGGC52950.014.0845965
GAACTCA196350.013.5978644
CACTAGC140000.013.0307214
AGAACTC200900.013.0300673
CTTTATT241150.013.0077371
TCACTAG141650.012.9463013
ACAACCG38250.012.9177782
TATTAGG59850.012.4623754
Produced by FastQC (version 0.11.2)