FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n02_3024_d5.352000000be445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n02_3024_d5.352000000be445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17784265
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA247060.13892055702048972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA159350.018.4224195
ATACTCG77950.018.12912274-75
GCCGATC78950.017.5081492-93
AGGTCGA169900.017.4206684
CGATTCG38700.016.68886418-19
ACCCAAC111900.016.3797916
CGCCATA86600.016.2923970-71
CGGCGCC125350.016.1636431
CGGCAAT29650.015.9178781
CGATCTT88150.015.64370294-95
CGCCGAC116100.015.4393941
TACTCGC91550.015.434751576-77
TCGCTCG41900.015.4211182
GTCGAAG195500.015.3504616
CGGCACT54350.015.3501691
TCGAAGT189950.015.29896457
CGCCGAT90500.015.01124292-93
AACCCAA154700.014.8001785
CCCGCGT95950.014.55519682-83
CGCCTCA84300.014.3060778
Produced by FastQC (version 0.11.2)