FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n02_3023_d5.352000000be4be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n02_3023_d5.352000000be4be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19898401
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA673490.33846438213804214No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC404320.2031922062481302No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA373630.18776885640207974No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC372300.18710046098678984No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG320120.1608772483778973No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG304570.15306255010138756No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA289320.1453986177080259No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG280800.1411168666266199No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG249560.12541711266146463No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG230430.11580327484605421No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC220880.11100389423250642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC170950.024.332076
GCCGATC143000.023.50142192-93
GGTCGAA310300.022.8861645
CGCCGAT146750.022.59337292-93
AGGTCGA317500.022.5200734
CGATCTT152150.022.02210294-95
GAAACCC190050.021.973663
ATACTCG161000.021.5229974-75
AACCCAA215900.021.3858835
TCGAAGT338100.021.150557
GTCGAAG346400.020.6301636
TACTCGC169250.020.40215776-77
CCGATCT163250.020.3051494-95
CAACCCT196650.020.0156979
CCCGCGT173050.019.73643382-83
CGCCATA180400.019.35542370-71
CCGCGTG179650.019.24932782-83
AAACCCA251200.019.0830824
CCAACCC218800.018.9225358
AGAAACC230150.018.6824232
Produced by FastQC (version 0.11.2)