FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n02_3014_d5.352000000be70e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n02_3014_d5.352000000be70e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17412949
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA426230.24477760774467322No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC259110.14880305455440085No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC250570.14389865840645374No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA244170.1402232327218095No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG215000.12347133159351699No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG190340.1093094570023722No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG178020.10223426255943206No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA175890.1010110349487614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAA197300.023.2091585
GCCGATC98550.022.09967692-93
ACCCAAC134400.021.9754666
AGGTCGA209350.021.9220474
CGCCGAT98950.021.626392-93
ATACTCG107550.020.73631374-75
TCGAAGT225600.020.4163047
CGATCTT107900.020.17655494-95
GTCGAAG230100.019.9965086
CCGATCT109800.019.653394-95
TACTCGC113300.019.55678476-77
AACCCAA176750.019.2156945
GAAACCC160950.018.9804153
CGCCATA118150.018.71680570-71
CCCGCGT119400.018.51944482-83
CAACCCT161800.017.9005269
CCGCGTG125600.017.77545782-83
AAACCCA212200.017.351354
CGCGTGG131300.016.8775384-85
TCAGGTC317600.016.8592362
Produced by FastQC (version 0.11.2)