FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n02_3011_d5.352000000be1b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n02_3011_d5.352000000be1b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15827703
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT44450.023.0592461
AACCGTG43750.022.0449142
ACCGTGT66450.014.7997653
GTATACG9750.013.1493666
CCGTGTT78500.012.6487574
AACTCAC97850.012.2335295
CGGAAAT34200.011.7093171
CGTGTTC89600.011.3983645
ACTCACC101850.011.0492556
CTCGGAT39450.010.9969641
CGGTAAT9600.010.9252121
TTCACTA93900.010.820949
TCACTAG76500.010.4955383
CGCAGAT49150.010.4755941
TATACGT13400.010.276357
CACTAGC74000.010.1437034
CGAAAAT27750.010.136021
CTTTATT172250.010.0190781
CGCCAAT22200.09.87832451
AATCGAG20200.09.87665755