FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n02_3005_d3.352000000be3cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n02_3005_d3.352000000be3cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18780001
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA717460.3820340584646401No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC490950.26142171131939773No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA482210.25676782445325746No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG422170.22479764511194647No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC414220.22056441850029723No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG362260.1928966883441593No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG293800.15644301616384365No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA286930.15278486939377692No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG285010.15176250523096352No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG253890.1351916860920295No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA243200.12949946062303191No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC240540.1280830602724675No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG232850.12398827880786588No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC214780.11436634108805425No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT212860.11334397692524086No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC208590.11107028162565061No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG202790.10798188988381845No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT198740.1058253404778839No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG195640.10417464834000807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC200600.027.9315536
GAAACCC220100.025.5782383
AACCCAA240600.024.5600195
CGCCGAT143000.024.41468892-93
GCCGATC144850.024.31602392-93
GGTCGAA315500.023.9989875
CAACCCT225800.023.9500039
AGGTCGA323450.023.5591854
CGATCTT152900.023.08777494-95
AAACCCA266300.023.0312824
AGAAACC253450.022.8316142
CCGATCT154500.022.61663494-95
CCCAACC246900.022.3281567
ATACTCG166050.022.11260674-75
GTCGAAG347100.021.9428886
TCGAAGT342150.021.9271537
CCAACCC252500.021.8688188
CAGAAAC283450.021.7305851
TACTCGC170000.021.52749476-77
CCCGCGT174700.020.80088282-83
Produced by FastQC (version 0.11.2)