FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n01_3035_d-2.351000000be5da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n01_3035_d-2.351000000be5da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17791222
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA1027350.5774476874044965No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC830590.4668538226323071No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA822170.4621211516555749No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG723220.4065038365549033No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC647580.3639884882556128No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG534830.30061453901255347No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA470820.26463612224050714No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG461180.2592177198395928No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG455210.2558621324606033No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC438280.24634620376273197No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG424250.23846029238463778No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT407780.22920291815817936No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG404510.22736493311139616No Hit
TTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCC404500.22735931236201762No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA397340.22333485580698165No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG381080.21419551731747263No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG368250.20698409586480346No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA366380.20593301573101613No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG364190.20470207161711546No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT342350.19242635497438007No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC330660.18585569895086465No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT325160.18276428679266662No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG315540.1773571258905094No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC312250.17550789934496913No Hit
CGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTG311110.17486713391581535No Hit
CAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCAT310790.174687269935702No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC308930.1736418105512932No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC295060.16584583116325569No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC287000.16131550716415097No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGACCGGTGTGGCCAATGCC285510.16047801550674823No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC284410.1598597330751086No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG280240.15751588058425667No Hit
CTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTC275120.15463805690244323No Hit
CAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCT272970.15342959578605672No Hit
CCGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT268890.1511363300396117No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC267630.1504281156179154No Hit
GGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGG267620.15042249486853684No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC266630.1498660406800612No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA266620.14986041993068266No Hit
GCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCCGGCAGG264170.14848333633293992No Hit
CCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCC263690.1482135403627699No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA263060.14785943315192177No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT261350.1468982850081911No Hit
GCGGAATTCACCCCCGCCGTCCACGCCTCCCTGGACAAGTTCTTCAGCGC260630.1464935910529361No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG258950.14554930515734107No Hit
CGCCGACAAGACCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACG251010.14108643015077885No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC250620.14086722092501572No Hit
CGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGAGCC250520.1408110134312303No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT249510.14024331774399756No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC246050.1382985384590221No Hit
GTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAG241600.13579730498557097No Hit
GTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGAC234260.13167167494172127No Hit
GTCAAGTCCACCTGGGGCAAGATCGGCGGCCACGCGGGCGAGTATGGCGG232380.1306149740585554No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT230920.12979434464928827No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA228580.1284790892947095No Hit
GCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTG228440.12840039880340992No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA225570.1267872437317684No Hit
AAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCAT222720.12518533015888397No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA222360.12498298318125646No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC222330.12496612093312084No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC218890.12303258314690245No Hit
CCAACGTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTAT216670.12178477678486616No Hit
CAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGCAGTTTGT216500.12168922404543095No Hit
GCAGCCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCAC210810.11849101764904062No Hit
CGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCCGGCAG209270.11762542224474518No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA206030.11580429944609762No Hit
CTCAGGTGCAGGCTGCCTATCAGAAGGTGGTGGCCGGTGTGGCCAATGCC205610.11556822797219887No Hit
CGGCCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTTG205590.11555698647344179No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC202800.11398879739682863No Hit
TGACTGGTGAGGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAAC202290.113702139178523No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG201170.1130726152481263No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG200680.11279719852857774No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG200670.1127915777791992No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC199580.11217891609693814No Hit
GAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGT199300.11202153511433897No Hit
CTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGT197470.11099293797806581No Hit
GTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTC197250.11086928149173789No Hit
GCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCA196500.11044772528834726No Hit
CACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAG195340.1097957183604364No Hit
TGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGC194600.1093797829064243No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC194160.10913246993376846No Hit
CTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCA191870.10784531832608239No Hit
TGAACGTGGACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTC190200.10690665317986589No Hit
GCAACCCCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGC188590.10600171252992065No Hit
AAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCA188480.1059398842867567No Hit
CTCTGAGCGACCTGCACGCCTACAAGCTGCGTGTGGACCCCGTCAACTTC188240.10580498630167168No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA188090.10572067506099356No Hit
CCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCTTGAA186370.10475390616788435No Hit
CTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAG183540.10316323409375702No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA179350.10080814010414799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA10800.036.05933
TAGGTCG10650.032.107773
ACCCAAC276950.031.484456
GAAACCC287300.029.8048763
AACCCAA301500.029.5666265
AAACCCA316100.028.6367154
CAGAAAC308900.028.3783071
AGAAACC304000.028.1988162
CAACCCT306350.027.9668439
CCCAACC311950.027.8454047
CCAACCC316250.027.3316368
AACTCAC69450.025.16525
GCCGATC210600.024.88782592-93
ACTCACC71250.024.7960726
CGCCGAT210800.024.3009192-93
CGATCTT215850.024.23865394-95
CCGATCT217950.023.52566194-95
GCGCTAA7150.022.5839613
TCCGCTA11850.022.0430452
ATACTCG258400.020.93750874-75
Produced by FastQC (version 0.11.2)