FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n01_3033_d8.351000000be547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n01_3033_d8.351000000be547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18870575
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA526630.2790746969819415No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC446020.23635739769455885No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA437910.23205970141344395No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG382370.20262763588284935No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC320530.1698570393323998No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG297590.15770054701565797No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG254300.13476006957922587No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG238840.12656742044161345No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA234090.12405027403775454No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG232500.12320769239941018No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC224830.11914316336412642No Hit
TTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCC218650.11586822341131629No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG214160.11348885765272124No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT207420.1099171593870351No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA202940.10754309288402712No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG194250.10293803977886207No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA191950.10171921099383564No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG191680.10157613109298472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC199350.023.6094386
CGCCGAT118950.022.800492-93
GCCGATC123200.022.49577392-93
GAAACCC219000.021.8383273
AACCCAA238350.021.1012925
CGATCTT133450.020.7679894-95
ATACTCG135300.020.7131674-75
CCGATCT132000.020.54632494-95
AAACCCA266600.020.09454
TACTCGC137250.020.05482176-77
CAACCCT227700.020.0441959
CCCAACC245450.019.252557
CCAACCC246300.019.2246728
GGTCGAA297150.019.211315
AGAAACC249300.019.0316832
AGGTCGA303300.018.7904454
CCCGCGT146350.018.78148582-83
CGCCATA151250.018.4193670-71
CCGCTAA14050.018.2537213
TCGCCCG157350.017.97715478-79
Produced by FastQC (version 0.11.2)