FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n01_3033_d3.351000000be263.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n01_3033_d3.351000000be263.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17949979
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG288310.16061857231142165No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC225100.12540404643370334No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA189790.10573271422768796No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC184990.10305861639169606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT68750.038.66491
AACCGTG73900.036.3107452
ACCGTGT98650.027.4415683
CCGTGTT112600.023.9571444
CGTGTTC121500.022.7494955
TTCACTA156200.017.452439
AACTCAC153450.016.1248595
TGTTCAC179450.016.0381497
GTTCACT175050.015.7087468
ACTCACC156750.015.57336
GTGTTCA187900.015.2663516
ACAACCG35600.015.2084862
ATTAGGC55350.014.58667855
CACTAGC145600.013.8628544
TCACTAG143200.013.5647393
GAACTCA197200.013.2458824
TATTAGG60200.013.0959414
ACTAGCA154750.012.9511075
TAGCAAC163650.012.2467697
AGAACTC200200.012.0512123
Produced by FastQC (version 0.11.2)