FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n01_3024_d5.351000000be448.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n01_3024_d5.351000000be448.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17784265
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA274370.15427682842107898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG46400.018.3219763
GCCGATC81650.018.03370392-93
ATACTCG82500.017.84840274-75
GGTCGAA184650.016.33275
CGCCATA93100.015.86747670-71
CGATCTT93600.015.73141394-95
TACTCGC93200.015.51858276-77
AGGTCGA195250.015.446014
CGCCGAT94350.015.10284492-93
CCGCGAT21900.015.0092611
CCCGCGT96750.015.002582-83
ACCCAAC113900.014.5941216
TCGAATC21050.014.4399332
GCTCGCG59750.014.3871355
CCGCGTG105350.014.27389682-83
TCGAAGT215350.014.1366587
GTCGAAG218650.014.0319066
CTCGGAT42650.013.96192551
CGCCGAC101850.013.8915191
CGCTCGC73300.013.8009584
Produced by FastQC (version 0.11.2)