FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n01_3023_d5.351000000be4b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n01_3023_d5.351000000be4b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19898401
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA730080.36690385322921176No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA377300.18961322570592481No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC374640.188276434875345No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC354260.17803440588015088No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG312260.15692718223941712No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA276260.13883527626164535No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG269510.13544304389081313No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG266980.13417158494293085No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG223880.1125115530639874No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG214940.10801872974617407No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC208270.10466670161084803No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG205240.10314396619105223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATC155100.024.54479692-93
CGCCGAT160750.023.62300792-93
ACCCAAC169450.022.8989146
GGTCGAA346450.022.8111525
CGATCTT168400.022.69103894-95
AGGTCGA353800.022.2701424
ATACTCG176250.021.88386774-75
CCGATCT175550.021.76685394-95
TACTCGC182200.021.11604376-77
TCGAAGT380600.020.8018157
GTCGAAG383650.020.5993046
GAAACCC186250.020.5022133
CCCGCGT186600.020.3948382-83
AACCCAA210200.020.2446235
CCGCGTG191700.019.96377682-83
CGCCATA195200.019.68680270-71
CAACCCT192100.019.1605859
CGCGTGG201500.018.94567584-85
ACTCGCC207650.018.61951676-77
TCGCCCG207150.018.59674578-79
Produced by FastQC (version 0.11.2)