FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n01_3014_d5.351000000be701.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n01_3014_d5.351000000be701.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17412949
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA455040.2613227661782045No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC244840.14060800384817068No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA243430.1397982616270225No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC241030.1384199769952809No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG205750.11815919290867963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC134950.022.3480036
CGCCGAT100150.020.55977892-93
GCCGATC101150.020.47391592-93
ATACTCG102450.020.28412874-75
CGACAAT13200.020.2117231
GGTCGAA215950.019.859695
TACTCGC107000.019.5986476-77
AACCCAA174650.019.1172145
AGGTCGA228050.018.8476144
GAAACCC159000.018.7887383
CGCGAAT5350.018.7005671
CGATCTT112450.018.56434694-95
CCGATCT113750.018.3939494-95
CCCGCGT116000.017.97994882-83
TCGAAGT244900.017.9193027
CGCCATA116750.017.88122470-71
CTCGAAT32700.017.7746621
CAACCCT158750.017.6811949
TCGCTCG33300.017.5430452
CCGCGTG123050.017.1814782-83
Produced by FastQC (version 0.11.2)