FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n01_3011_d5.351000000be1b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n01_3011_d5.351000000be1b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15827703
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT45250.024.0073591
AACCGTG49800.021.5534362
ACCGTGT64250.017.3712943
CCGTGTT77900.014.6321144
AACTCAC101950.013.4164795
CGTGTTC87300.012.9478025
ACTCACC103200.012.93182856
TTCACTA96200.011.8980369
CGAAAAC30500.010.7789721
GTTCACT112150.010.6717248
GAACTCA141650.010.5615424
CGGGAAT33600.010.4935041
CGACAAT15550.010.4178141
CGCGTAT4601.1537618E-410.3578651
TGTTCAC130600.09.8550637
CTCGAAT30950.09.8525221
CGGAAAT34600.09.7771051
CACTAGC76700.09.7215974
CCGCAAT17900.09.5824721
CGCGAAT6554.501311E-69.4564931
Produced by FastQC (version 0.11.2)