FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n01_3009_d8.351000000be66f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n01_3009_d8.351000000be66f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17127501
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA594650.3471901709420423No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC501080.2925587334661373No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA500400.2921617111568115No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG431650.2520215879712983No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC365370.21332358993877742No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG331190.19336738033178336No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG309970.18097794885546936No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA289050.16876367428032846No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG280110.16354399862536864No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG276120.16121441183976576No Hit
TTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACCC269600.15740766852093602No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC262860.1534724768079126No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT241650.141088883894971No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA236360.13800028387095117No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG230490.1345730471713299No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA230160.13438037458003943No Hit
CAGAATTTAGGGAAACAGGGTCTTCCAGTGGTCACCAGGAAATAGGCCAG220800.12891547926343722No Hit
GGAGAAGGCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAG212000.1237775434956915No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG209030.12204349017407734No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT207040.12088161606296213No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG206860.12077652192225825No Hit
CGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTG199460.11645598502665391No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC195040.1138753400160362No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC191340.11171507156823403No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT191320.11170339444148915No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC191000.11151656041357112No Hit
CAGCACCTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCAT183670.10723689346157388No Hit
CAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCT182140.10634359326559081No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC181510.1059757637731272No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC174110.10165522687752289No Hit
CTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTC173090.10105969341353417No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC172060.10045832138617303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC199000.026.1572936
GAAACCC216300.024.4167923
AACCCAA228850.024.177445
GCCGATC133050.022.91972292-93
AAACCCA253250.022.8044414
CGCCGAT131250.022.79976892-93
CAACCCT228850.022.392679
AGAAACC241400.022.1731342
CGATCTT140500.021.63682494-95
CCCAACC246300.021.5003627
CCAACCC244500.021.3672758
CAGAAAC267450.021.287861
CCGATCT142600.021.03505794-95
TAGGTCG11850.020.4405333
ATACTCG154750.020.19682974-75
CCGCTAA13500.020.0530973
GGTCGAA319500.019.74075
TACTCGC155750.019.70069776-77
AGGTCGA326050.019.3878274
CCCGCGT162050.018.83798482-83
Produced by FastQC (version 0.11.2)