FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l07n01_3005_d3.351000000be3cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l07n01_3005_d3.351000000be3cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18780001
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA780580.41564428031713097No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA480840.25603832502458335No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC458880.24434503491240497No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG404930.21561766690001774No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC400890.21346644230743117No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG349010.18584131065807719No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA266750.14203939605754015No Hit
CCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGACCTTGGGG265090.1411554770417744No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG259630.13824812895377375No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG230430.12269967397765315No Hit
CGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAG229900.12241745887020986No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA226740.12073481785224612No Hit
CGCACACCAGCACGTTGCCCAGGAGCTTGAAGTTCTCAGGATCCACGTGC224520.11955270928899313No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG214700.11432374258127036No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT201820.10746538298906375No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC199240.10609158114528322No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG192160.10232161329490877No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC191590.10201809893407353No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT190990.10169861013319435No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT189180.10073481891720878No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC189150.10071884447716484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCAAC192050.025.990786
CGCCGAT155550.025.52797392-93
GCCGATC158650.025.26867392-93
TAGGTCG13950.024.5129173
CGATCTT167350.024.01189494-95
GGTCGAA350100.023.7940485
AACCCAA231100.023.5513385
CCGATCT170550.023.4499694-95
GAAACCC208150.023.4331513
AGGTCGA361450.023.1388634
ATACTCG180700.022.43562374-75
TACTCGC184150.022.04018276-77
TCGAAGT383600.021.7903867
AAACCCA255150.021.7223194
GTCGAAG384750.021.6388516
CAACCCT224850.021.5234649
CCCGCGT187350.021.42921382-83
CCCAACC239100.020.7571457
CCGCGTG195800.020.69853282-83
CCAACCC239600.020.5948968
Produced by FastQC (version 0.11.2)