FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n02_3034_d3.352000000be27d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n02_3034_d3.352000000be27d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13095053
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG235230.17963272084503973No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC217040.165741979051173No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC171560.13101130633071895No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA148210.1131801452044524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT58300.042.9490471
AACCGTG59700.041.380592
ACCGTGT78650.031.2275453
CCGTGTT89150.027.7606184
CGTGTTC98950.025.0080535
TTCACTA118900.020.6058799
TGTTCAC135150.018.9714727
GTTCACT130900.018.9707878
GTGTTCA141450.018.2275586
AACTCAC136000.017.7761125
ACTCACC138800.017.0019426
GAACTCA161050.015.4260154
ACAACCG27600.015.319642
AGAACTC164600.014.459523
CTCAGAA166800.014.4122481
ATTAGGC33100.013.9187965
AGACTCA90000.013.6172674
TCACTAG109200.013.616573
CACTAGC112600.013.5418944
CTTTATT158200.013.0893821
Produced by FastQC (version 0.11.2)