FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n02_3032_d3.352000000be198.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n02_3032_d3.352000000be198.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14075599
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG173020.12292194456520109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT54450.035.1469351
AACCGTG58600.032.6719362
ACCGTGT72700.026.137943
CCGTGTT81700.023.4894374
CGTGTTC93400.020.9000035
TTCACTA105800.018.4452259
GTTCACT119150.016.3389368
TGTTCAC128000.015.4312767
GTGTTCA138950.014.7279866
AACTCAC97900.013.4869435
ACTCACC100500.012.7089846
GTATACG8500.012.2893366
CGGAAAT26750.012.1016451
CGGGAAT28800.011.9014341
CACTAGC93050.011.58396810-11
ACAACCG28550.011.1490062
CGGCAAT22950.010.99391651
TCACTAG92050.010.9410213
CTTTATT153300.010.9309971
GAACTCA132550.010.7511484
Produced by FastQC (version 0.11.2)