FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n02_3030_d8.352000000be3a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n02_3030_d8.352000000be3a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13664180
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG1456551.0659622458135065No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT848820.6212008331271983No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA804220.5885607478824195No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC756920.553944693351522No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC730500.5346094679666106No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC601090.4399019919234085No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA575250.4209912340147744No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC570020.4171637083235145No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG540490.3955524590571845No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG529010.38715093038879755No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT490440.35892384321635107No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC488440.3574601622636704No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC487780.3569771475492858No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA459340.3361636044021668No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA447620.32758643401945814No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG442310.32370036109009104No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA426470.3121080079448602No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA426130.3118591821829045No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA420990.3080975221345152No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC412490.3018768780856224No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC410630.3005156547996294No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA407310.2980859444181795No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT382920.2802363552002389No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC380980.2788165846761386No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC376010.2751793375087272No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG374800.2742938105323554No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG362560.2653360831019498No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT360200.2636089395777866No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG349870.256049027457191No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC346550.2536193170757411No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG338700.24787436933646953No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC329090.24084138235883895No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT324520.23749687138196365No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA320780.2347597880004508No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT317590.23242521688092516No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA312320.22856841757061164No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA306390.22422860354591348No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA303460.2220843109502363No Hit
AAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACAC297010.2173639398778412No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT296950.21732002944926077No Hit
CTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGA293390.2147146773534892No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC289220.21166290256715No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC286580.20973084370961154No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA286460.20964302285245073No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT285200.2087209038522619No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA284610.20828911797122113No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC283240.20728649651863487No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG279790.2047616468752607No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC277410.20301986654157073No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG277330.2029613193034635No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA270710.19811653535009052No Hit
GTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCA270430.19791162001671522No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC268850.1967553120640975No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA266180.19480129799226883No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC263160.19259113975372105No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG260030.1903004790627758No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA256550.18775367420511146No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT254120.18597530184760447No Hit
CCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTC250910.183626093918552No Hit
CTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTCCACCTG247680.18126224917997275No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC244440.1788910860366301No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA243210.1779909222507315No Hit
CTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCT234310.17147754201130255No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA233400.17081156717783283No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG228180.16699135989133632No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT224670.1644225998193818No Hit
AGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCAC220600.16144400908067663No Hit
CTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCT220400.16129764098540858No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA216920.15875083612774424No Hit
ATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCT214080.1566724091749377No Hit
CAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGC213130.15597716072241438No Hit
CTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGA212610.15559660367471742No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC212230.15531850429370805No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA211240.15459398222213117No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC208460.15255946569790502No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG208410.152522873674088No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC204350.14955160134014628No Hit
AGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGC204310.14952232772109267No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT202570.1482489252922605No Hit
CTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAG199070.14568748362506934No Hit
CCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACT197470.14451653886292481No Hit
GTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTG195530.14309676833882456No Hit
CTGTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGG193400.14153794812421966No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC191740.1403230929334947No Hit
AACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGG191240.13995717269532457No Hit
ATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGC184740.13520020959911241No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC184590.13509043352766137No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT183560.13433663783703084No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG181150.13257290228905064No Hit
CGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC179830.13160687286028142No Hit
CCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGC179180.1311311765506602No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG173130.12670354166880118No Hit
TGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTG170430.12472757238268231No Hit
CTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTATCAGAAGGTGG168770.12351271719195736No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA168720.12347612516814035No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC166030.12150747428678486No Hit
CCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTG165920.12142697183438741No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA164020.12003647492934082No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC158890.1162821332857149No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC157920.11557224802366478No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT157790.11547710876174055No Hit
CCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCC157640.1153673326902895No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG157430.11521364619025805No Hit
CGGCAGGTCGTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCT156390.11445253209486408No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA155880.11407929345193053No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC155640.11390365173760883No Hit
GTGGACTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGT155260.1136255523565995No Hit
CCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGAT153690.11247656280874521No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA151400.11080064811792585No Hit
CTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGCGGTGACGG151250.11069087204647479No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG150150.10988584752250044No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA150040.10980534507010299No Hit
CGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGA148140.10841484816505637No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG145990.10684139114092467No Hit
CCCACTCAGACTTTATTCAGAGATCGGGAGGTTGAGGTGCGCGAAGCGCA145390.10640228685512047No Hit
CCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGCCCCACAGGGC145010.10612418747411115No Hit
CAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGACCA144160.10550212306922185No Hit
CTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGA142810.10451413842616242No Hit
TTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAG142180.10405307892606801No Hit
CCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGAC142120.1040091684974876No Hit
GTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCC141570.10360665623550042No Hit
GAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCA140540.10285286054486986No Hit
GCTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTG140150.10256744275909714No Hit
CAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGC139460.1020624728304223No Hit
GACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTG138160.10111108021117989No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC136870.10016700599670086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT242950.059.078051
ACAACCG92100.056.0712782
AACCGTG264750.054.3004572
ACAGACT113250.052.4375422
CACAGAC124750.048.6166041
CACAACC113300.045.840631
ACCGTGT317000.045.1526573
CCGTGTT338500.042.2953834
AGACTCA152250.039.654884
CGTGTTC362950.039.4018485
CAGACTC156850.038.1921423
GTGTTCA419050.034.2307556
TGTTCAC422600.033.897647
CTCAGAA252450.030.6808871
CGGGTCT29500.029.85421
TCAGAAA257300.029.6719862
CGCCCTT31200.029.6007611
GTTCACT503950.028.4546138
TTCACTA508550.028.178359
CAGAAAG284000.026.7970893
Produced by FastQC (version 0.11.2)