FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n02_3026_d5.352000000be2f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n02_3026_d5.352000000be2f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13806118
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC323400.23424397792341048No Hit
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG320210.23193340807314555No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC251880.18244085701715718No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA210330.15234550363831456No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA165720.12003374156297955No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC162030.11736101342897402No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT150500.10900964340591612No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC150070.10869818728189923No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA143990.1042943425516137No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG141370.10239663314481305No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC140340.10165058708030744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT68400.047.256971
AACCGTG71100.045.0348852
ACCGTGT92500.035.025353
CCGTGTT105600.030.6333644
CGTGTTC118200.027.4044845
TTCACTA146450.022.0796749
TGTTCAC152700.021.4241757
GTGTTCA157300.021.1903746
GTTCACT156400.020.9485328
ACAACCG30250.020.8874342
AACTCAC174550.019.3465925
ACTCACC179850.018.7446846
AGACTCA103950.017.4562264
GAACTCA202400.017.3438474
ACAGACT98300.017.1566942
AGAACTC202600.017.1402763
ATTAGGC41700.016.9709035
CTCAGAA184000.016.5323751
TATTAGG46550.015.2047074
TCACTAG136900.014.3313283
Produced by FastQC (version 0.11.2)