FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n02_3022_d5.352000000be203.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n02_3022_d5.352000000be203.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13999462
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG236750.16911364165280066No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC188020.1343051611554787No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC150390.10742555678211062No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA148580.10613264995469113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT56550.040.2371141
AACCGTG59000.038.166972
ACCGTGT76150.030.1315483
CCGTGTT93400.024.6661724
CGTGTTC101700.022.7901255
AACTCAC125950.018.289065
TTCACTA125350.018.0686979
ACTCACC128000.017.805976
GTTCACT136550.016.5519668
TGTTCAC141950.016.457277
ACAACCG30850.016.0154652
GTGTTCA153800.015.68345456
ATTAGGC39900.015.3555245
GAACTCA158400.015.3540054
TCACTAG110800.014.7061363
AGAACTC162750.014.6238253
CACTAGC111500.014.4421464
CGCGAAT4352.2710083E-814.2260931
CTTTATT180100.013.5592361
ACTAGCA118600.013.49562555
Produced by FastQC (version 0.11.2)