FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n02_3021_d3.352000000be161.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n02_3021_d3.352000000be161.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13198055
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT43400.029.7270261
AACCGTG47600.026.7486212
ACCGTGT64650.019.9135153
CCGTGTT73200.017.9755784
CGTGTTC82350.016.3217265
TTCACTA93250.013.6969769
TCACTAG81000.013.1960733
CACTAGC80750.012.9417914
ACAACCG26600.012.859512
AACTCAC89950.012.6722375
GTTCACT106500.012.3942828
TGTTCAC118300.012.2013667
ACTCACC95900.012.1303146
ACTAGCA86200.012.1215655
CGACGAT9200.011.901781
CGACAAT12000.011.901781
CGCGAAT4405.6731133E-611.9017791
GTGTTCA128500.011.2329816
TAGCAAC94450.011.0596237
CTAGCAA98100.010.69665056
Produced by FastQC (version 0.11.2)