FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n02_3012_d3.352000000be062.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n02_3012_d3.352000000be062.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14773899
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT47750.028.712261
AACCGTG52000.026.0382632
ACCGTGT67450.020.5657653
CCGTGTT73800.018.4729234
CGTGTTC84000.016.6798045
TTCACTA97150.014.3203039
CTCGTAT15600.014.0372311
CACTAGC85600.013.0962924
TCACTAG87100.013.035383
CGGCAAT23200.012.5167071
GTTCACT117100.012.2861168
CGCGAAT5101.8178434E-712.1344881
AACTCAC97100.012.1305535
ACTAGCA93300.011.9119385
TGTTCAC126500.011.8233777
ACTCACC103600.011.5495936
TAGCAAC98200.011.2659017
GTGTTCA134250.011.2470536
AGACTCA95200.010.927414
CCGGAAT30900.010.6301371
Produced by FastQC (version 0.11.2)