FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n02_3010_d3.352000000be02f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n02_3010_d3.352000000be02f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12872842
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG259160.20132306447946774No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA178280.13849311597237035No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC149140.11585631207156896No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC147590.11465222675769654No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT134940.10482533693802815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT65050.043.0314671
AACCGTG69300.039.96712
ACCGTGT82200.033.6359183
CCGTGTT96550.028.732754
CGTGTTC107800.025.9064255
ACAACCG30100.021.4654372
TTCACTA133450.020.9920859
TGTTCAC145650.019.8209157
GTTCACT144500.019.617288
GTGTTCA154500.018.5627576
AACTCAC110600.014.7294995
TCACTAG121300.014.4124943
CACTAGC119900.014.3418774
ACTCACC112700.014.0720226
ATTAGGC36850.013.7909835
AGACTCA89950.013.4665274
ACTAGCA131200.013.28565
CTTTATT163650.012.4504161
GAACTCA141050.012.3597474
TAGCAAC139750.012.2994987
Produced by FastQC (version 0.11.2)