FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n02_3007_d3.352000000be438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n02_3007_d3.352000000be438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15015381
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG463270.308530299697357No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA313250.20861941498520753No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC303610.2021993314721751No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC295900.197064596629283No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT271610.1808878509309887No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA198300.13206458097866447No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC195790.13039296172371517No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC190750.1270364035384783No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG182420.12148875876010073No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG174530.11623414683916446No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA170630.11363681014820737No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG170350.11345033469347197No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT169190.11267779352385396No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC159560.10626436984849069No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA158040.10525207452278433No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA157750.10505893923037984No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA155850.10379357007324688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT93600.049.3848651
AACCGTG103250.044.9093252
ACCGTGT128200.036.130063
CCGTGTT142600.032.7450374
CGTGTTC157650.029.796255
ACAACCG42500.029.7351042
TGTTCAC199700.023.6815517
TTCACTA206800.022.5240929
GTGTTCA213350.022.2782176
GTTCACT215050.021.81478
AGACTCA112700.020.4844284
ACAGACT115050.019.0366732
AACTCAC185250.017.7421885
ATTAGGC51450.017.5399155
ACTCACC191050.016.9254066
CTCAGAA208650.016.9063661
TATTAGG54400.016.8527164
CACAACC85250.015.7472671
GAACTCA215000.015.730884
CACAGAC156700.015.5543351
Produced by FastQC (version 0.11.2)