FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n01_3034_d3.351000000be270.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n01_3034_d3.351000000be270.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13095053
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG244680.18684918648286494No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC205910.1572425861888455No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC165410.12631487631245172No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA158230.12083188972201946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT56250.043.630881
AACCGTG60300.040.6421432
ACCGTGT81800.030.4244143
CCGTGTT90450.027.6719424
CGTGTTC102400.024.8136925
TTCACTA123950.020.269679
CGTATGC31900.019.2711811
GTTCACT133800.019.2034288
TGTTCAC138600.018.7439757
TATGCCG32500.018.7055643
ACAACCG26300.018.4199582
GTGTTCA145300.017.9450366
AACTCAC126050.017.8219785
ACTCACC130500.016.9231056
ATTAGGC35850.015.7650525
GAACTCA154550.015.0886394
CGCGTAT3806.619848E-815.0489751
AGAACTC157550.013.9273263
TATTAGG41250.013.7012634
CTTTATT170300.013.5437831
Produced by FastQC (version 0.11.2)