FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n01_3032_d3.351000000be19b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n01_3032_d3.351000000be19b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14075599
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG177650.12621132500293591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTG53400.031.752352
CAACCGT56000.030.9780141
ACCGTGT72250.023.9283313
CCGTGTT83450.020.7166424
CGTGTTC95000.018.8478585
TTCACTA103400.016.9492369
CGCGTAT4352.2362656E-814.2427651
GTTCACT123550.014.1849548
TGTTCAC133300.013.9669077
AACTCAC94250.013.7066665
ACAACCG29150.013.3605462
GTGTTCA144050.013.32025056
ACTCACC98650.012.8064526
CCGGTAT13750.012.131251
CGACAAT14050.011.5330131
CGCAAAT17800.011.5130041
CACTAGC92450.011.3021244
ATTAGGC31100.011.3009665
TCACTAG93000.011.1332643
CGGGAAT29950.010.9797511
Produced by FastQC (version 0.11.2)