FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n01_3029_d1.351000000be53a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n01_3029_d1.351000000be53a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14214882
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG1535041.0798823373982283No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA1090660.7672663058335624No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC898180.6318589208127088No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC777910.5472504098169791No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT752430.5293255336203283No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT603560.4245972636283579No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA593050.4172036039412779No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC560030.3939744276456182No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG551450.38793849994674595No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG544680.3831758856668666No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC541380.3808543750134542No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG535090.37642943501043485No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA477320.3357889288141822No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA468590.32964747790379123No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC456230.32095236527464666No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC444150.31245422930700373No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA444080.31240498514162834No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG443300.31185626444173087No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA437840.30801521954244854No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA437620.30786045216555435No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA429340.30203557088971966No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC425890.2996085370247885No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC424310.2984970258634577No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC402210.28294993936636265No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC388160.2730659318874402No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG386480.2718840719184303No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA380520.2676912829807521No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC362690.25514809057155735No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT361200.2540998933371378No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA357230.251307045672275No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA353550.2487182095496818No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG350440.2465303616308598No Hit
AAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACAC337800.23763827234021356No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG334500.2353167616868012No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT328940.2314053679798397No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC326820.22991397325704144No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT321370.22607996323852705No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC317760.22354037128130927No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC317500.22335746438134343No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT314180.2210218839663952No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA313110.22026915172422817No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC311770.2193264777013274No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT309840.21796874571311953No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT309770.21791950154774412No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA309620.2178139783362254No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC308480.21701200192868292No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA308080.21673060669796626No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC303250.21333275928706266No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG299370.21060322554911112No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT298710.21013892341842866No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA295180.2076556105073542No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA285750.2010217179432091No Hit
CCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTC284430.20009311368184413No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA284230.1999524160664858No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA281300.19789119600148636No Hit
GTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCA275230.1936210233753611No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG271170.1907648617835871No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG269940.1898995714491334No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG269520.1896041064568809No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC268020.18854887434169343No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT265130.18651579379976563No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC264300.18593189869602855No Hit
TTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAG260830.1834907950695616No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA260250.18308277198502246No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC259830.18278730699276996No Hit
CTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGA259820.18278027211200207No Hit
CTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCT258280.18169690047374296No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA257000.18079643573544965No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC248660.17492934517500744No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA245720.17286109022924004No Hit
CTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTCCACCTG244420.17194655572941092No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG236680.16650155801504368No Hit
AGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCAC231490.1628504548964951No Hit
ATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCT230410.16209068777356012No Hit
CAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGC227720.16019830484699066No Hit
TGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTG226830.1595722004586461No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC223980.15756725943978994No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT223250.15705371314373204No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC221250.15564673699014878No Hit
GTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTG219710.15456336535188966No Hit
CTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGA216990.15264987778301642No Hit
CGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC216830.15253731969072976No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG215920.15189714554084938No Hit
CTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCT212200.1492801698951845No Hit
CCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGC210910.1483726702761233No Hit
AGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGC210700.14822493777999704No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC206240.14508738095750637No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG205980.14490447405754056No Hit
CCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACT205820.14479191596525387No Hit
CCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTG203620.14324424219631227No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT203450.1431246492232577No Hit
CTGTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGG194360.13672994260522175No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA192250.13524558276319143No Hit
ATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGC190440.13397226934419856No Hit
CTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAG189260.13314215341358446No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA189190.13309290924820905No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC188720.13276226985211695No Hit
CGGCAGGTCGTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCT188260.1324386653367928No Hit
AACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGG185160.13025785229873876No Hit
TTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCA182020.12804889973761302No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC180260.12681076072245975No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA177980.12520680790737482No Hit
CAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGC177820.12509424981508815No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC177560.12491134291512233No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG174950.12307523903469617No Hit
CAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGACCA173630.12214663477333122No Hit
CCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCC173460.12202704180027664No Hit
GCTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTG170650.12005024030449214No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC166390.1170533810973598No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG166130.11687047419739398No Hit
AGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCA165740.11659611384744523No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT162930.11461931235166076No Hit
GTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTC161140.11336006869420372No Hit
TGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGAC159800.11241739467130293No Hit
CCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGAT159650.11231187145978418No Hit
CGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGA159590.11226966217517668No Hit
TTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTT159570.11225559241364086No Hit
CTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTATCAGAAGGTGG159290.1120586157521392No Hit
GATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCC159280.11205158087137128No Hit
CGGCGCTGAAGAACTTGTCCAGGGAGGCGTGGACGGCGGGGGTGAATTCC158290.11135512767534758No Hit
TGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTA156030.10976524462179849No Hit
GAAGAATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGC155270.10923059368343684No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT153990.10833012894514356No Hit
GGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAAGGTTCTCTCCAGAG153610.10806280347596271No Hit
GAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCA153580.10804169883365897No Hit
CGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCGCGCTGTCC151230.10638850185319863No Hit
CTCCGCCCAGGTCAAGGCCCACGGCAAGAAGGTGGCCGACGCGCTGACCA149930.10547396735336953No Hit
TGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTAT149910.10545989759183369No Hit
GTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCAT148740.10463681654198749No Hit
CACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAA148230.10427803762282374No Hit
CGGCCACCTTCTTGCCGTGGGCCTTGACCTGGGCGGAGCCGTGGCTCAGG147890.10403885167671459No Hit
CTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGA147460.10373635180369419No Hit
GACGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTG147120.10349716585758503No Hit
CCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGAC146170.10282885218463299No Hit
CCCACTCAGACTTTATTCAGAGATCGGGAGGTTGAGGTGCGCGAAGCGCA145930.102660015046203No Hit
GTTCTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCC145700.1024982127885409No Hit
CAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAA145590.10242082910009384No Hit
CTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTA145360.10225902684243175No Hit
CACGGCTCCGCCCAGGTCAAGGCCCACGGCAAGAAGGTGGCCGACGCGCT144670.10177362006944551No Hit
GTGGACTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGT143640.10104902735035015No Hit
GCAGCCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCAC143340.10083798092731266No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA142500.10024705094280768No Hit
CGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGG142350.10014152773128893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT230050.065.3211441
AACCGTG253850.059.441872
ACAACCG72050.054.1863942
ACAGACT104450.051.5197682
CACAGAC111250.048.623691
ACCGTGT322000.046.7432373
CACAACC84350.046.6144941
CCGTGTT349350.043.5998044
CGTGTTC379950.040.100915
CGCCCTT27900.039.2894331
AGACTCA144400.038.219524
CAGACTC151000.036.046253
CGGGGTT18750.034.8234181
GTGTTCA443450.034.4229056
TGTTCAC447500.034.1007547
CTCAGAA242100.031.7141321
CGGGTCT27950.030.8637621
TCAGAAA250650.030.375142
CGCCGAA7750.028.288391
GTTCACT548750.027.7915068
Produced by FastQC (version 0.11.2)