FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n01_3026_d5.351000000be2f7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n01_3026_d5.351000000be2f7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13806118
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG333590.24162476374604358No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC312120.2260736870422229No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC239710.17362592439091135No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA222910.16145740605722766No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA183950.13323803258816128No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC156940.11367424210049486No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC146510.10611962030166626No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT145050.10506211811314375No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG143980.10428709938593891No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC142870.1034831079960348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT66800.048.2307781
AACCGTG71850.044.5537262
ACCGTGT97000.033.3936273
CCGTGTT111050.029.296564
CGTGTTC124250.026.566425
ACAACCG28250.020.847512
TGTTCAC162100.020.8320127
TTCACTA160050.020.6537179
GTGTTCA168300.020.0081426
GTTCACT169900.019.4283548
AACTCAC169400.019.0090715
ACTCACC172250.018.2533846
ACAGACT97100.017.0220152
AGACTCA103650.017.0000134
TCACTAG153100.016.8141543
CACTAGC154900.016.710514
GAACTCA198100.016.6387084
ACTAGCA162700.015.9093935
AGAACTC197250.015.8678963
ATTAGGC42600.015.1626095
Produced by FastQC (version 0.11.2)