FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n01_3022_d5.351000000be206.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n01_3022_d5.351000000be206.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13999462
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG250700.17907831029506704No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC178750.12768347812223071No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA158960.11354722060033451No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC144110.10293967011017996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT59600.042.3016741
AACCGTG63000.039.9564742
ACCGTGT84500.030.127283
CCGTGTT99850.025.971094
CGTGTTC109550.023.5414435
TTCACTA134450.019.0049659
TGTTCAC145150.018.3565657
GTTCACT143200.018.1090328
GTGTTCA158350.016.9763376
AACTCAC121550.016.3330365
ACTCACC124200.015.7168636
ACAACCG28600.015.4443182
ATTAGGC40250.014.3959255
GAACTCA155000.014.1871414
TATTAGG44400.013.15733054
TCACTAG120800.013.0140623
CACTAGC119500.012.8772384
AGAACTC161200.012.7577673
AGACTCA93900.012.6956164
ACTAGCA126750.012.3280265
Produced by FastQC (version 0.11.2)