FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n01_3021_d3.351000000be164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n01_3021_d3.351000000be164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13198055
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG133940.1014846505791952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT46000.030.150291
AACCGTG46800.029.8364032
ACCGTGT63500.022.1392523
CCGTGTT71400.020.1554
CGTGTTC80650.017.5489145
TTCACTA94250.015.3190069
AACTCAC88450.014.6052645
GTTCACT108700.013.457358
ACTCACC92000.012.9575916
TGTTCAC116550.012.9177057
TCACTAG81950.012.1127373
CACTAGC79950.012.0591284
GTGTTCA128350.011.6560946
ACTAGCA84500.011.6346155
CTCGGTT35600.011.51343251
GAACTCA121300.011.2763894
ACAACCG23400.011.163282
CTTTATT143650.011.0814651
TAGCAAC93000.010.418037
CTCGATT16600.010.33595851
Produced by FastQC (version 0.11.2)