FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n01_3012_d3.351000000be065.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n01_3012_d3.351000000be065.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14773899
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT44400.031.2356511
AACCGTG52300.026.2450982
ACCGTGT72850.018.515733
CCGTGTT77000.017.5792484
CGTGTTC87250.015.9495455
TCACTAG90900.014.6300633
TTCACTA103200.014.0369322
CACTAGC93100.013.7740054
ACTAGCA100400.012.6305915
GTTCACT120850.012.5012431
TAGCAAC105550.012.0593177
CTAGCAA112600.011.3042716
TGTTCAC129950.011.2203987
AACTCAC95350.011.0082365
GTGTTCA140600.010.7758496
ACAACCG28750.010.5729012
CGACAAT14900.010.55524351
TATCGCG4601.1915065E-410.3249599
CTTTATT161100.010.2062021
AGACTCA98500.010.1740154
Produced by FastQC (version 0.11.2)