FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n01_3010_d3.351000000be022.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n01_3010_d3.351000000be022.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12872842
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG270490.21012453970925765No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA188090.14611381076533062No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC140190.10890369042049922No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC137220.10659650759327272No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT134870.1047709588915952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT63550.043.7979931
AACCGTG66250.042.2264062
ACCGTGT92000.030.5624773
CCGTGTT102100.027.7248024
CGTGTTC110400.025.8555225
ACAACCG30800.022.0515962
TTCACTA141100.020.2299779
TGTTCAC152000.019.497957
GTTCACT152400.019.0104688
GTGTTCA164000.018.1581526
AACTCAC109700.014.9368975
ACTCACC111550.014.4337126
TCACTAG129300.014.3625963
CACTAGC130600.014.3285084
ATTAGGC36350.013.7193275
AGACTCA88100.013.3158524
ACTAGCA141800.013.0628015
CTTTATT167900.013.0008491
TAGCAAC148300.012.4262057
CTAGCAA148950.012.3082066
Produced by FastQC (version 0.11.2)