FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l06n01_3007_d3.351000000be43b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l06n01_3007_d3.351000000be43b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15015381
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG491830.32755079608036586No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA332730.22159277876465472No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC294420.1960789406542531No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC276690.18427104846690204No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT264070.17586633332847165No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA195000.12986683454785464No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC191310.12740935444794907No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC189200.12600412869976457No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG188100.1252715465561613No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG183200.12200822609829214No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT182220.12135556200671832No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG167860.11179203511386092No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA164580.10960760835838931No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA163700.10902154264350668No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA163370.10880176800042571No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC161330.10744316111592506No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA152550.10159582364243705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT97050.048.462191
AACCGTG104600.044.7710042
ACCGTGT132900.035.5590173
CCGTGTT147150.032.695924
ACAACCG44100.031.4482082
CGTGTTC163800.029.6043995
TGTTCAC214000.023.0593057
GTGTTCA221950.022.468736
TTCACTA217050.022.1882299
GTTCACT227400.021.3036568
ACAGACT109550.020.506922
AGACTCA120750.019.6271764
AACTCAC181000.016.7149565
CACAACC89400.016.5236341
ACTCACC186400.016.1542856
TCACTAG206700.016.084563
CACAGAC153350.016.03421
CTCAGAA204600.015.8840091
ATTAGGC55400.015.8601175
CACTAGC211200.015.6516374
Produced by FastQC (version 0.11.2)