FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l05n02_3030_d1.352000000be2cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l05n02_3030_d1.352000000be2cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13243095
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG1097000.8283562112935081No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA832240.6284331570527886No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC786200.593667869935238No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC742640.5607752568413954No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT659070.4976706729053895No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG529420.3997705974320957No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC521080.3934729759168835No Hit
CTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACA506570.38251632265720364No Hit
CTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCTTGA486150.36709696638134814No Hit
CTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCACGTGGATCCTG468530.35379191948709876No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC443810.33512558808949117No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG443390.33480844168225027No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA432520.32660039061865825No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC426810.3222887097011688No Hit
CTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTTATGAGCAACC425630.3213976793189205No Hit
CTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGA421040.3179317221540735No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA417840.31551536857509516No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT413180.311996553675708No Hit
CTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAA396450.2993635551206119No Hit
CTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACAAACTGCA396410.2993333507008747No Hit
CGACAAACTGCACGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGC378230.285605441930304No Hit
CAAGAAGGTGCTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACC356290.2690383177044339No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG338350.25549163545228665No Hit
GTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAG326770.24674745593835884No Hit
GCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGA320880.24229985513205182No Hit
CTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGG315070.23791266316521933No Hit
CTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCT312310.2358285582033505No Hit
CCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACC308850.23321587589608017No Hit
CTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAG308790.23317056926647436No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC302880.22870786625029874No Hit
CTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGT301570.22771867150390449No Hit
CCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCA301390.22758275161508695No Hit
GTCAACTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCA288770.21805325718799118No Hit
CTTGAAGTTGACGGGGTCCACACGCAGCTTGTAGGCGTGCAGGTCGCTCA287490.21708671575639982No Hit
GGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGACA285980.21594649891131945No Hit
CTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGT284630.21492709974518798No Hit
CGACCAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACC283050.21373402516556744No Hit
GTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCC282270.21314503898069145No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA280080.21149134700007818No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT280060.21147624479020952No Hit
CCTGCACCTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCAC269850.2037665666522818No Hit
CTGAACTCCTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCAC268160.2024904299183839No Hit
CTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGA264890.20002121860486538No Hit
CCGGTCACCACCTTCTGATAGGCAGCCTGCACCTGAGGGGTGAATTCCTT264380.19963611225321576No Hit
CGTGGATCCTGAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGC263290.19881304181537623No Hit
CTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATGCTGTT263110.1986771219265587No Hit
AAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACAC261830.19771058049496737No Hit
CACAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGAC260550.19674403906337606No Hit
CTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTCCACCTG260320.19657036364988698No Hit
CTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTCCTCTCCTGATG259410.19588321310086504No Hit
CTGGTGTGCGTGCTGGCTCACCACTTCGGCAAGGAATTCACCCCTCAGGT259190.19571708879231026No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGTCACCACCTTC258080.19487891614460215No Hit
CCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTCCCCA257520.19445605426828094No Hit
CTTTATTAGGCAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAG256860.19395768134261665No Hit
GCCAGGGTCACCAGCAGGCAGTGGCTCAGGAGCTTGAAGTTGACGGGGTC251370.18981212473368198No Hit
CTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGCGA250610.18923824075867463No Hit
GTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTC247870.18716923800667443No Hit
CTCATAACAGCATCAGGAGAGGACAGGTCCCCAAAGGAGTCAAAGAACCT246530.18615738994547723No Hit
GAGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCA238450.18005609715855697No Hit
CCTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTC235340.1777077035239874No Hit
CTCAGTGGTACTTGTGAGCCAGGGCATTGGCCACACCGGCCACCACCTTC231020.17444562619236667No Hit
GTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCA225920.17059456267586995No Hit
CTTGACCTGGGCGGAGCCGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCT224410.16945434583078955No Hit
GTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAG222410.1679441248439281No Hit
CTTTAGCGAGGGCCTGAAGAATCTGGACAACCTCAAGGGCACCTTCGCTA221040.166909623467928No Hit
GGACAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGA221030.1669020723629937No Hit
GCTGCCGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGG217890.16453102541362122No Hit
GTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCA214210.16175221879779614No Hit
CGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGA209710.15835422157735785No Hit
CTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGCT207900.15698747158424825No Hit
CAGAAACCCAACCCTTGATGGTGGACTTTATTTCCCCCAGTTCAGAAGAC204790.15463907794967868No Hit
AGAACTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCAC203690.15380845640690488No Hit
CAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGG192720.1455248942939698No Hit
GGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCT192560.1454040766150209No Hit
CAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGCCGTCAC192160.1451020324176486No Hit
ATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGCCT191770.1448075393252106No Hit
GTTTGTCGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTG191550.14464141501665584No Hit
GTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTGTC186860.14109994680246574No Hit
TGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAGAGGACAGGTC186800.1410546401728599No Hit
CTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGGCAAGA186420.14076769818535623No Hit
CAGCAGCCTGCCCAGGGTCTCACCACCAACTTCGTCCACGTTCACCTTGC185520.14008809874126857No Hit
CTTCAAGCTCCTGAGCCACTGCCTGCTGGTGACCCTGGCCTGCCACCACC184440.13927257940836338No Hit
CTCAGAGCGGACAGCGCGCCCGGCAGGTCGTCCATGTGGGCCACGGCGTT183130.13828338466196913No Hit
CCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCACGTTGCCCAGGAGC182280.13764154074255303No Hit
CCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTCAAGGCCCACGGCAAG181910.13736214985998363No Hit
CCTGGGCAGGCTGCTGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACT179360.1354366181017353No Hit
CGTCACCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGA177000.13365455733723877No Hit
CGTGGCTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGAC176820.13351863744842124No Hit
GGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCACGCCTACA176170.13302781562769128No Hit
CCCACATGGACGACCTGCCGGGCGCGCTGTCCGCTCTGAGCGACCTGCAC175490.13251434049215838No Hit
CCGCCCTGTGGGGCAAGGTGAACGTGGACGAAGTTGGTGGTGAGACCCTG173090.13070207530792463No Hit
CGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCCCACATGGACG170740.1289275656483624No Hit
AGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGGGC169980.12835368167335506No Hit
CTTCGCGTCCTTCCCCACCACCAAGACCTACTTCCCCCACTTCGACCTGA167340.12636018997069792No Hit
CTTCTTGCCATGGGCCTTGACCTTGGGGTTGCTCATAACAGCATCAGGAG166180.12548426179831831No Hit
CGGCAGGTCGTCCATGTGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCT161800.1221768778370917No Hit
CCACACGCAGCTTGTAGGCGTGCAGGTCGCTCAGAGCGGACAGCGCGCCC161170.12170115822623036No Hit
TTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAG160520.12121033640550037No Hit
TGGTCGTCTACCCCTGGACTCAGAGGTTCTTTGACTCCTTTGGGGACCTG159180.12019848834430319No Hit
CTGTCCTCTCCTGATGCTGTTATGAGCAACCCCAAGGTCAAGGCCCATGG158740.11986623972719368No Hit
GTCAAGTCCACCTGGGACAAGATCGGCGGCCACGCGGGCGAGTATGGCGG154160.11640783366728094No Hit
ATCTGGACAACCTCAAGGGCACCTTCGCTAAGCTCAGTGAGCTGCACTGC153830.11615864720444882No Hit
CAGGTCCCCAAAGGAGTCAAAGAACCTCTGAGTCCAGGGGTAGACGACCA152020.11479189721133919No Hit
CAGGAAATAGGCCAGGGTCTCAGTGGTACTTGTGAGCCAGGGCATTGGCC149950.1132288184899376No Hit
CTCCGCCCAGGTCAAGGCCCACGGCAAGAAGGTGGCCGACGCGCTGACCA146470.11060103397279866No Hit
CCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTG145570.10992143452871099No Hit
CCAAGGTCAAGGCCCATGGCAAGAAGGTGCTGAACTCCTTTAGCGAGGGC141130.10656874393787857No Hit
CGCAGTGCAGCTCACTGAGCTTAGCGAAGGTGCCCTTGAGGTTGTCCAGA140920.10641017073425812No Hit
TTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCCA139990.10570791797536754No Hit
GGGTCTTCCAGTGGTCACCAGGAAATAGGCCAGGGTCTCAGTGGTACTTG139900.10563995803095877No Hit
GGGAGGTTGAGGTGCGCGAAGCGCAAGAGGGCCCCGGGCCGGGAGCCCGA139630.10543607819773247No Hit
CAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGGCTGC138370.10448463897600975No Hit
CGGAGAGGCTCTGGAGAGAACCTTCGCGTCCTTCCCCACCACCAAGACCT138340.10446198566120685No Hit
CGCTGACCAACGCCGTGGCCCACATGGACGACCTGCCGGGCGCGCTGTCC138030.1042279014082433No Hit
CTTCGGCAAGGAATTCACCCCTCAGGTGCAGGCTGCCTATCAGAAGGTGG136170.10282339589046216No Hit
GTGAATTCCGCGGGGTGGTGGCAGGCCAGGGTCACCAGCAGGCAGTGGCT135830.10256665832269571No Hit
GTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCAT135460.10228726744012635No Hit
CTTAGCGAAGGTGCCCTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAA134860.10183420114406791No Hit
CTGAGGGGTGAATTCCTTGCCGAAGTGGTGAGCCAGCACGCACACCAGCA133990.10117725501478318No Hit
GTGGACTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGT133790.10102623291609703No Hit
GCTTGAAGTTCTCAGGATCCACGTGCAGTTTGTCGCAGTGCAGCTCACTG133440.10076194424339627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT171700.064.216191
AACCGTG185400.059.5024382
ACAACCG54700.049.789372
ACCGTGT230950.047.7193573
ACAGACT97350.047.554582
CCGTGTT251100.043.8867034
CACAGAC108200.043.4153981
CGTGTTC273400.040.2995875
CACAACC70950.038.8677751
AGACTCA128100.036.725274
GTGTTCA321700.034.463276
TGTTCAC322800.034.2281577
CAGACTC138050.034.1152883
CTCAGAA231650.033.804571
TCAGAAA241050.031.8050882
CGCCCTT28600.030.2565541
CAGAAAG264550.028.760433
CGGGTCT28650.028.0463391
GTTCACT403450.027.3750828
AGAAAGA280950.027.1302784
Produced by FastQC (version 0.11.2)