FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l05n02_3028_d5.352000000be12e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l05n02_3028_d5.352000000be12e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13234519
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG390360.29495594059746333No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC290030.21914661197736013No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC256260.19363000650042514No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA230240.17396929952648826No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC165870.12533133996029625No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT155450.11745798997303944No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT155200.11726909002132983No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA145460.10990954790272317No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG142460.10764274848220777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT74650.050.8271641
AACCGTG81500.046.2487642
ACCGTGT108350.035.177573
CCGTGTT118100.032.070384
CGTGTTC132550.028.6037835
ACAACCG33300.024.8364522
TGTTCAC174500.022.1759877
TTCACTA174900.021.6116739
GTGTTCA184700.021.3369316
GTTCACT182900.020.8729028
AACTCAC167950.018.8123025
ACTCACC175750.017.8577566
ACAGACT96800.017.8244362
AGACTCA103500.017.4018154
GAACTCA201350.016.096394
CTCAGAA188200.015.7142471
AGAACTC203100.015.6310043
TCACTAG164200.014.7899983
CACTAGC163250.014.75878054
ACTAGCA172450.013.8581455
Produced by FastQC (version 0.11.2)