FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l05n02_3023_d3.352000000be500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l05n02_3023_d3.352000000be500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14145162
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG284570.2011783251404261No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC262990.1859222255637652No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC224520.1587256476808113No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA165360.11690216061152214No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC144540.10218334721086969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT67500.041.6312981
AACCGTG72600.038.104372
ACCGTGT88650.031.0942963
CCGTGTT100900.027.2701854
CGTGTTC113300.024.7841245
TTCACTA137550.020.4375789
TGTTCAC149150.019.4511267
ACAACCG31050.019.1353682
GTTCACT150750.018.8673178
AACTCAC163900.018.3791875
GTGTTCA158800.018.2990156
ACTCACC171300.016.9359936
AGACTCA111100.015.7838524
GAACTCA195200.015.7759644
ACAGACT105250.015.5354832
AGAACTC201750.015.2648643
CTCAGAA185950.013.5523911
TCACTAG126850.013.525343
CACTAGC125450.013.4859024
ATTAGGC38900.012.9470945
Produced by FastQC (version 0.11.2)