FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l05n01_3028_d5.351000000be121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l05n01_3028_d5.351000000be121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13234519
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG413760.3126369760774835No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC276170.20867399865457897No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA244400.18466859279132095No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC243610.18407166894391855No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT175130.13232819417162045No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC159780.12072973713665No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT155150.1172313100309879No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG153870.11626414227823467No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA136090.10282957771264675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT78900.052.0159341
AACCGTG86850.047.081552
ACCGTGT114750.035.675663
CCGTGTT131400.031.1551154
CGTGTTC144200.028.6201555
ACAACCG34650.028.0975232
TGTTCAC185250.022.4832367
TTCACTA185700.022.2242439
GTGTTCA193900.021.7251746
GTTCACT196900.020.839498
AACTCAC158800.018.1832085
ACTCACC159200.017.7795456
AGACTCA105550.016.2430064
ACAGACT96400.016.158992
CTCAGAA176600.015.4388731
GAACTCA189450.015.2414554
CACAACC73200.014.7166051
AGAACTC194350.014.71056653
TCACTAG176500.014.1533263
CACTAGC177850.013.9390784
Produced by FastQC (version 0.11.2)