FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l05n01_3023_d3.351000000be503.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l05n01_3023_d3.351000000be503.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14145162
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG300130.2121785526387043No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC252340.17839314954469945No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC210300.14867274054549534No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA176960.12510284435059846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT64850.043.882451
AACCGTG69050.040.7168582
ACCGTGT92400.030.5302733
CCGTGTT107550.026.4504244
CGTGTTC119600.023.8251865
TTCACTA139950.020.2590569
TGTTCAC155250.018.9354347
GTTCACT152050.018.740568
GTGTTCA162650.018.307536
ACAACCG31700.017.8280542
AACTCAC152150.016.9177885
ACTCACC158400.016.52016
GAACTCA183450.014.7302734
ACAGACT100750.014.4242292
AGACTCA109650.014.4228744
CTCAGAA184700.014.11714651
TCACTAG132550.013.7942233
ATTAGGC41100.013.750595
AGAACTC188300.013.7203393
CACTAGC132200.013.6151994
Produced by FastQC (version 0.11.2)