FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n02_3032_d5.352000000be650.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n02_3032_d5.352000000be650.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13393096
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG212570.15871610268454733No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC145300.10848873180629781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT59050.037.415561
AACCGTG59900.036.6381532
ACCGTGT78150.027.959713
CCGTGTT88250.024.8111974
CGTGTTC99100.022.1858565
TTCACTA113000.019.2398419
GTTCACT126450.017.7200628
TGTTCAC131250.017.5080157
GTGTTCA142600.016.547576
ACAACCG30250.014.7611742
AACTCAC112500.014.6891125
ACTCACC118150.013.8236276
AGACTCA95300.012.9581184
TCACTAG101400.012.5076893
CGACAAT12250.012.4117531
CGGAAAT24600.012.3612991
GAACTCA145750.012.0248394
CACTAGC103950.011.89644310-11
CGCGGTT15600.011.8784351
CTCAGAA168850.011.0870091
Produced by FastQC (version 0.11.2)