FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n02_3026_d3.352000000be23a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n02_3026_d3.352000000be23a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15204859
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG170150.11190501667920762No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC159050.10460471879416967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT56650.031.7889251
AACCGTG55250.031.641222
ACCGTGT74850.024.1166293
CCGTGTT82250.022.0603374
CGTGTTC98600.018.445935
TTCACTA105000.017.0888849
AACTCAC122900.015.4556345
ACTCACC126000.014.7712396
GTTCACT124350.014.4686358
TGTTCAC133550.014.2560447
CGCAAAT17350.013.9671321
GTGTTCA143050.013.3757636
GAACTCA161100.012.5602634
AGAACTC166250.012.0008623
CGGAAAT32100.011.9899251
CGCGAAT5202.3971188E-711.8789081
CCGGAAT35850.011.663531
ACAACCG30550.011.5068962
CACTAGC90700.010.88645810-11
TCACTAG90300.010.73166753
Produced by FastQC (version 0.11.2)