FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n02_3016_d5.352000000be6d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n02_3016_d5.352000000be6d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11581580
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG333560.28800906266675186No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC319120.27554098836255503No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC271060.2340440596188085No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA199900.17260166574854208No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC162100.13996363190514594No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT149240.12885979287800112No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA147190.12708974077802856No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA139670.12059667161130003No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC136680.1180149858654864No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG128730.11115063747778801No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT128060.11057213264511405No Hit
GAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGT123630.10674709322907583No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA123490.10662621162224842No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC122670.10591819078225942No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG117600.10154054973501025No Hit
CCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCTTCTTGCCATGG116930.10096204490233629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT62250.053.1250341
AACCGTG68850.047.814642
ACCGTGT89550.036.4956633
CCGTGTT99900.032.9961664
CGTGTTC112300.029.261735
ACAACCG26350.026.3210872
TGTTCAC147500.022.6927977
TTCACTA147450.022.4392099
GTGTTCA153400.022.3466366
GTTCACT152900.021.8585998
ACAGACT86350.019.529792
AACTCAC169950.019.4753485
CTCAGAA166350.019.051641
ACTCACC175000.018.5845856
AGACTCA96950.018.4208454
GAACTCA191500.017.1883434
ATTAGGC39300.016.7956145
AGAACTC197350.016.704783
TCAGAAA182850.016.4712242
TATTAGG41100.015.8324564
Produced by FastQC (version 0.11.2)