FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n02_3012_d5.352000000be7bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n02_3012_d5.352000000be7bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12766963
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT34950.023.111731
AACCGTG36850.021.786092
ACCGTGT52100.015.6822053
CCGTGTT61700.013.7025634
CGTGTTC71500.012.4859985
CGACAAT12950.012.1080691
CGGGAAT26300.011.381921
TTCACTA73800.011.0631359
ACTCACC86350.010.9416756
AACTCAC80800.010.8725615
CGGAAAT28350.010.726491
CGCGTAT3550.001675643710.7076051
CGCATAT7151.1599695E-810.6327281
CGCGGTT14750.010.6304751
GTCGATT10800.010.5588891
CTCGGAT33900.010.5121671
CTCGATT16350.010.1714061
GTTCACT84950.010.0589128
CGAAGAT46000.010.0194671
CTTGGAT87000.09.9945341
Produced by FastQC (version 0.11.2)