FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n02_3009_d3.352000000be2a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n02_3009_d3.352000000be2a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13341874
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG153880.11533612144740686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT43850.034.1312181
AACCGTG50350.029.2470192
ACCGTGT65900.022.056963
CCGTGTT75900.019.211614
CGTGTTC83000.018.0225565
CACTAGC89850.014.7486844
TCACTAG93550.014.6237343
AACTCAC104050.014.4221145
TTCACTA101900.014.3580672
ACTCACC107900.014.3450956
ACTAGCA96450.013.736815
GTTCACT114700.013.3383861
TGTTCAC122050.012.6817427
TAGCAAC104050.012.6397017
CTAGCAA108000.012.265576
GTGTTCA131500.012.2038886
ACAACCG26950.011.6333442
GAACTCA136750.011.3576264
AGAACTC136550.011.3405873
CTTTATT154550.011.0058821
Produced by FastQC (version 0.11.2)