FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n02_3008_d3.352000000be1e8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n02_3008_d3.352000000be1e8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14799466
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG306840.20733180507999407No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC210600.14230243172287432No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC180760.12213954206185547No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA170810.11541632650799699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT72000.042.7628361
AACCGTG73800.041.3883932
ACCGTGT93300.032.6862833
CCGTGTT110550.027.7123534
CGTGTTC120950.025.5198675
ACAACCG30900.021.061342
TTCACTA147850.020.9330489
GTTCACT158600.019.8748978
TGTTCAC164600.019.3829177
GTGTTCA174550.018.795196
AACTCAC138800.016.524515
ACTCACC146100.015.7608666
GAACTCA173300.014.1424144
AGACTCA111850.013.2492424
AGAACTC185250.012.8979873
TCACTAG136900.012.76899053
CACTAGC136950.012.5550654
CTCGTAT15950.012.5115931
CTCAGAA186500.012.2798261
CTTTATT185050.012.1963131
Produced by FastQC (version 0.11.2)