FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n02_3002_d5.352000000be13b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n02_3002_d5.352000000be13b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13237418
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG172520.13032753064079416No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC165710.12518302285234173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT48800.032.9104651
AACCGTG50750.031.5445292
ACCGTGT70500.022.9090373
CCGTGTT79700.020.024434
CGTGTTC90700.017.7492945
AACTCAC115850.016.3965595
ACTCACC117550.015.7932136
TTCACTA103950.015.5721739
GTTCACT115100.014.1059728
GAACTCA146300.013.6359114
TGTTCAC124750.013.62544257
GTGTTCA129300.013.476716
AGAACTC153300.013.1383483
ACAACCG28650.012.9330272
ATTAGGC34650.012.1976315
TCACTAG91550.011.7783533
CACTAGC90400.011.6644354
AGACTCA96300.011.640324
CCGGTCT51950.011.1586451
CGACAAT12250.010.8607331
Produced by FastQC (version 0.11.2)