FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n01_3032_d5.351000000be653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n01_3032_d5.351000000be653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13393096
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG226320.1689825862519017No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC139120.10387441410111599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT60050.037.4691
AACCGTG62600.036.0338252
ACCGTGT80100.028.7541333
CCGTGTT93400.024.7612844
CGTGTTC103200.022.5479725
TTCACTA119250.019.5530369
TGTTCAC135600.017.8608287
GTTCACT130250.017.8288048
GTGTTCA149750.016.3951266
ACAACCG30500.016.1928982
CGCGATT3755.6059434E-815.254331
AACTCAC111250.014.6841735
ACTCACC113100.014.2760286
CACTAGC104000.012.7854434
TCACTAG107400.012.4249063
GAACTCA146600.012.2123284
AGACTCA92950.011.7508564
ACTAGCA115850.011.3956695
AGAACTC151000.011.3532793
CTTTATT156300.011.2845921
Produced by FastQC (version 0.11.2)