FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n01_3026_d3.351000000be23d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n01_3026_d3.351000000be23d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15204859
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG176780.11626546487540595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT58850.033.2085231
AACCGTG60600.031.6596052
ACCGTGT76850.025.0269493
CCGTGTT88150.021.9264774
CGTGTTC97650.020.0364955
TTCACTA111000.017.7978329
AACTCAC116100.015.6252845
GTTCACT130250.015.2039038
ACTCACC119900.014.8528176
TGTTCAC141950.014.4191167
GTGTTCA151150.013.7614046
CCGGTAT15550.012.874521
GAACTCA154500.012.3872054
CGGAAAT32950.012.151671
CGAGAAT27450.011.8080641
GTATACG10300.011.5265566
CACTAGC95950.011.4578410-11
CGGGAAT29050.011.3217911
AGAACTC161650.011.3104993
CTTTATT165650.011.2799391
Produced by FastQC (version 0.11.2)