FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n01_3016_d5.351000000be6d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n01_3016_d5.351000000be6d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11581580
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG352430.3043021763869869No Hit
AAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTCAAGTC303210.2618036571866706No Hit
CTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAACGTC253600.21896839636733503No Hit
GTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGA215080.18570868568882656No Hit
CTCAGGTCGAAGTGGGGGAAGTAGGTCTTGGTGGTGGGGAAGGACGCGAA160030.13817631100419803No Hit
CACAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACC154560.13345329393744204No Hit
CTTGAGGTTGTCCAGATTCTTCAGGCCCTCGCTAAAGGAGTTCAGCACCT143590.12398135660246702No Hit
ACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGT143450.12386047499563964No Hit
CTAGCAACCACAAGGAGACACCATGGTGCATCTGACTGGTGAGGAGAAGG141920.12253941172102598No Hit
CCAAGACCTACTTCCCCCACTTCGACCTGAGCCACGGCTCCGCCCAGGTC133080.11490660168992488No Hit
CAGACTCAGAAAGAACTCACCATGGTGCTGTCTCCCGCCGACAAGACCAA132870.11472527927968378No Hit
CTTTATTTCCCCCAGTTCAGAAGACAGAATTTAGGGAAACAGGGTCTTCC126260.10901794055733328No Hit
CGCCGCTTCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACGGCGC125670.1085085109285607No Hit
CGAAGTTGGTGGTGAGACCCTGGGCAGGCTGCTGGTCGTCTACCCCTGGA123410.10655713641834706No Hit
CTTCAAGCTCCTGGGCAACGTGCTGGTGTGCGTGCTGGCTCACCACTTCG117300.10128151772038012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT65050.050.7075461
AACCGTG70500.046.682062
ACCGTGT94150.035.359293
CCGTGTT107050.031.0539784
CGTGTTC115900.028.6007795
ACAACCG27750.026.5261732
TGTTCAC153800.022.20137
TTCACTA151500.021.7860289
GTGTTCA157650.021.6289946
GTTCACT160100.020.9123888
ACAGACT85300.019.152052
AACTCAC162550.019.1071835
AGACTCA96750.019.0452484
ACTCACC164300.018.8169546
ATTAGGC39200.017.8089035
GAACTCA183850.017.3068124
CTCAGAA161950.017.129941
AGAACTC187150.017.0523933
TATTAGG43450.015.6297534
CACAGAC121800.015.1061511
Produced by FastQC (version 0.11.2)