FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n01_3012_d5.351000000be7be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n01_3012_d5.351000000be7be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12766963
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT34250.021.99041
AACCGTG38950.019.2639452
ACCGTGT51700.014.1457443
CCGTGTT61300.012.2403054
AACTCAC75400.012.02977855
CGTGTTC67650.011.2317595
CCGGAAT28350.011.097561
ACTCACC79800.011.009426
TTCACTA68550.010.7379129
CCGCGAT16150.010.3307561
CTCGGAT32550.010.2514121
CTCGAAT21000.09.9878041
CTCGGTT34650.09.7676871
CGGGAAT25650.09.6639041
TCACTAG56650.09.6403673
CCGGTCT49850.09.5625011
CGAGAAC43000.09.5338131
CGGGCAT36200.09.2177741
GTTCACT83450.09.1621038
CGAAAAC23150.09.0602111