FastQCFastQC Report
Tue 13 Feb 2018
CBNFWACXX_l04n01_3009_d3.351000000be2a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBNFWACXX_l04n01_3009_d3.351000000be2a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13341874
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCGTGTTCACTAGCAACCACAAGGAGACACCATGGTGCATCTGACTG162200.12157212697406676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGT45500.035.7234881
AACCGTG51100.031.319542
ACCGTGT67150.023.975083
CCGTGTT78050.020.991944
CGTGTTC84150.019.6395475
TTCACTA104500.015.5877789
CACTAGC93400.014.8471244
TCACTAG95100.014.5317813
CGCGTAT3303.815363E-614.4443131
GTTCACT116900.014.1780778
TGTTCAC123400.013.8930777
ACTAGCA102150.013.6683265
CGCGAAT5009.615178E-913.3465471
GTGTTCA136700.012.8540386
CTTTATT156400.012.7699181
TAGCAAC109150.012.7047317
AACTCAC96150.012.59497455
ACAACCG26850.012.5579992
ACTCACC105500.012.0639276
CTAGCAA115100.012.0067116
Produced by FastQC (version 0.11.2)